Validation of transport measurements in skeletal muscle with N-13 amino acids using a rabbit isolated hindlimb model

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(89)90585-7· OSTI ID:6149931

Conlon, K C; Bading, J R; DiResta, G R; Corbally, M T; Gelbard, A S; Brennan, M F

The authors are studying the transport of C-11 and N-13 labeled amino acids in tumor-bearing rabbits to determine the role of amino acid transport in the pathogenesis of muscle wasting in cancer. To validate a new, in vivo, method for measuring transport in skeletal muscle with these compounds, an isolated hindlimb model was developed in rabbits. The limb was perfused with a non-recirculating, normothermic, constant pressure system and a cell-free perfusate. Hemodynamic and metabolic parameters were measured during the first 75 min. of perfusion and found to remain normal and stable. Flow varied directly with perfusion pressure over the normal range of resting flows in the intact rabbit hindlimb. Time-activity curves (TAC's) were recorded from the medial thigh following bolus co-injection of L-(amide N-13) glutamine or N-13 L-glutamate with Tc-99m human serum albumin (HSA) into the femoral artery. Regional plasma flow was determined from the Tc-99m data.

• Research Organization: Memorial Sloan-Kettering Cancer Center, New York, NY (USA)
• OSTI ID: 6149931
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 44:13; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MODELS
BLOOD VESSELS
BODY
CARBON 11
CARBON ISOTOPES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANE TRANSPORT
MINUTES LIVING RADIOISOTOPES
MUSCLES
NEOPLASMS
NITROGEN 13
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PERFUSED TISSUES
PROTEINS
RABBITS
RADIOISOTOPES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TESTING
TISSUES
TRACER TECHNIQUES
VALIDATION
VERTEBRATES
YEARS LIVING RADIOISOTOPES