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Glutathione redox status of control and cadmium oxide-exposed rat lungs during oxidant stress

Journal Article · · J. Toxicol. Environ. Health; (United States)
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In this study, the ability of the lung to maintain levels of glutathione (GSH) during challenge with tert-butyl hydroperoxide (tBuOOH) was evaluated in isolated perfused lungs from control and cadmium oxide-exposed rats. Changes in glutathione redox status were indicated by measurements of nonprotein sulfhydryls (NPSH), total glutathione (1/2 GSH + GSSG), and glutathione disulfide (GSSG) in liquid nitrogen freeze-clamped lungs after 3-min infusions with 0-0.6 mM tBuOOH. In control and cadmium oxide-exposed lungs, levels of 1/2 GSH + GSSG remained constant over the range of 0-0.6 mM tBuOOH, indicating that no loss of glutathione from the system had occurred. In experiments with control lungs, levels of NPSH fell from 8.04 +/- 0.22 to 3.09 +/- 0.40 ..mu..mol/g dry weight when tBuOOH concentrations were increased from 0 to 0.6 mM (n = 20-23). In cadmium oxide-exposed lungs, NPSH levels also decreased proportionally to increases in GSSG. However, at concentrations of 0.075 and 0.15 mM tBuOOH, significantly smaller decreases in NPSH levels were observed in cadmium oxide-exposed lungs compared with controls. This protection against the GSH-depleting effects of tBuOOH might be explained by increased tissue levels of GSH-related enzymes.

Research Organization:
Johns Hopkins Univ., Baltimore, MD (USA)
OSTI ID:
7090127
Journal Information:
J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 22:3; ISSN JTEHD
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
BODY
CADMIUM COMPOUNDS
CADMIUM OXIDES
CHALCOGENIDES
DRUGS
ENZYME ACTIVITY
GLUTATHIONE
KINETICS
LUNGS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXIDIZERS
OXYGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
TOXICITY
VERTEBRATES