Influence of selenium deficiency on rat cardiac glutathione metabolism during hydroperoxide infusion

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5382005

Taylor, M A; Hill, K E; Burk, R F

Selenium-deficient (O Se) rats have decreased cardiac glutathione peroxidase (GSH-Px) activity. As a result, hydroperoxides cannot be detoxified effectively. The authors examined the effect of t-butylhydroperoxide (BuOOH) infusion on cardiac glutathione (G) pools in O Se and control (C) isolated perfused hearts. Hearts from male rats were perfused with oxygenated Krebs-Henseleit buffer by the Langendorff method. After 20 min, 30 ..mu..M BuOOH was infused for 20 min. The heart was freeze-clamped and intracellular GSH, GSSG and GSH-protein mixed disulfide (PS-SG) concentrations were determined. Recoverable G from hearts perfused with buffer only was 1180 + 180 nmol/g and 1340 +/- 95 nmol/g for C and O Se, respectively. C hearts had a 65% decrease in GSH. This was accounted for by GSSG and PS-SG formation and GSSG release to the perfusate. BuOOH infusion into the O Se heart did not increase intracellular GSSG, but decreased GSH by 24%. The fall in GSH was not accounted for by PS-SG or by GSH or GSSG release to the perfusate. These results suggest that there is a mechanism other than GSH-Px by which GSH and hydroperoxides interact in the O Se heart.

• Research Organization: Univ. of Texas Health Science Center, San Antonio
• OSTI ID: 5382005
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARDIOVASCULAR SYSTEM
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLUTATHIONE
HEART
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INFUSION
INTAKE
MAMMALS
METABOLISM
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEPTIDES
PERFUSED ORGANS
PEROXIDASES
PEROXIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SELENIUM
SEMIMETALS
VERTEBRATES