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Glutathione: metabolism and interactions with sulfur dioxide in the lung

Thesis/Dissertation ·
OSTI ID:6544067
The effect of SO/sub 2/ and sulfite on lung cells was investigated using a novel HPLC system to separate and quantitate glutathione (GSH), glutathione disulfide (GSSG), glutathione S-sulfonate (GSSO/sub 3/H) and cysteine S-sulfonate (CYSSO/sub 3/H). Cultured human lung cells, grown under conditions to maintain constant GSH levels, formed GSSO/sub 3/H when exposed to 0.5 mM sulfite or greater. GSH increased and GSSG decreased concurrently. Rat lung cells in culture also formed GGSO/sub 3/H when exposed to 5 mM sulfite or greater, with no change in GSH or GSSG. Sulfite did not decrease the viability of the cells significantly, nor was GSSO/sub 3/H overtly toxic to A549 cells. The time course of GSSO/sub 3/H formation was studied. SO/sub 2/ did not have as noticeable an effect on rat lung glutathione as sulfite did in cultured cells. The glutathione status was altered as 10 ppm SO/sub 2/ after four and twelve hours of exposure. No GSSO/sub 3/H was detected after SO/sub 2/ exposure. To assess the importance of GSSO/sub 3/H in lung cells, a mathematical model was constructed to determine the effect GSSO/sub 3/H could have on conjugation of BaP metabolites in lung cells.
Research Organization:
Duke Univ., Durham, NC (USA)
OSTI ID:
6544067
Country of Publication:
United States
Language:
English

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Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
CELL CULTURES
CHALCOGENIDES
CHROMATOGRAPHY
DRUGS
GLUTATHIONE
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MAMMALS
MAN
MATHEMATICAL MODELS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SEPARATION PROCESSES
SULFITES
SULFUR COMPOUNDS
SULFUR DIOXIDE
SULFUR OXIDES
TOXICITY
VERTEBRATES
VIABILITY