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Title: Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid

Abstract

The cell line designated PIR-2 is a nonimmunogenic X-ray-induced thymoma of C57BL/6 origin that is unable to induce antitumor immunity in syngeneic lymphocytes in vitro and in mice in vivo. Fusion of PIR-2 with an allogeneic universal fuser A9HT (clone 3c) resulted in the establishment of a somatic cell hybrid designated A9/PIR. C57BL/6 lymphocytes sensitized in vitro with A9/PIR could lyse parental PIR-2 cells, as well as other syngeneic tumors. However, immunization of mice with the hybrid significantly enhanced PIR-2 tumor takes while it partially protected the animals against a challenge with unrelated syngeneic tumors. The results imply that somatic cell hybridization can increase the immunogenicity of an otherwise nonimmunogenic tumor. However, in view of the enhancing effects of hybrid preimmunization on parental tumor cell growth, the possible application of this approach for immunotherapy is questionable.

Authors:
; ;
Publication Date:
Research Org.:
The Hebrew University-Hadassah Medical School, Jerusalem, Israel
OSTI Identifier:
7088734
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Natl. Cancer Inst.; (United States); Journal Volume: 68:5
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HYBRIDIZATION; IMMUNOTHERAPY; LYMPHOMAS; RADIOINDUCTION; IMMUNITY; LYMPHOCYTES; X RADIATION; ANIMAL CELLS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LEUKOCYTES; MATERIALS; NEOPLASMS; RADIATIONS; SOMATIC CELLS; THERAPY; 550602* - Medicine- External Radiation in Diagnostics- (1980-); 551000 - Physiological Systems; 560121 - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Yefenof, E., Goldapfel, M., and Ber, R.. Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid. United States: N. p., 1982. Web.
Yefenof, E., Goldapfel, M., & Ber, R.. Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid. United States.
Yefenof, E., Goldapfel, M., and Ber, R.. 1982. "Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid". United States. doi:.
@article{osti_7088734,
title = {Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid},
author = {Yefenof, E. and Goldapfel, M. and Ber, R.},
abstractNote = {The cell line designated PIR-2 is a nonimmunogenic X-ray-induced thymoma of C57BL/6 origin that is unable to induce antitumor immunity in syngeneic lymphocytes in vitro and in mice in vivo. Fusion of PIR-2 with an allogeneic universal fuser A9HT (clone 3c) resulted in the establishment of a somatic cell hybrid designated A9/PIR. C57BL/6 lymphocytes sensitized in vitro with A9/PIR could lyse parental PIR-2 cells, as well as other syngeneic tumors. However, immunization of mice with the hybrid significantly enhanced PIR-2 tumor takes while it partially protected the animals against a challenge with unrelated syngeneic tumors. The results imply that somatic cell hybridization can increase the immunogenicity of an otherwise nonimmunogenic tumor. However, in view of the enhancing effects of hybrid preimmunization on parental tumor cell growth, the possible application of this approach for immunotherapy is questionable.},
doi = {},
journal = {J. Natl. Cancer Inst.; (United States)},
number = ,
volume = 68:5,
place = {United States},
year = 1982,
month = 5
}
  • Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping formore » constructing high-resolution, contiguous maps of mammalian chromosomes.« less
  • ASL-1 x LM(TK)/sup -/ somatic hybrid cells form both H-2/sup a/ and H-2/sup k/ antigen complexes. After forming a localized tumor in syngeneic (A/J x C/sub 3/H/HeJ)F/sub 1/ mice, they are rejected. Such mice are resistant to otherwise invariably lethal injections of ASL-1 cells, surviving for prolonged and, in some instances, indefinite periods. To examine the basis of immunity, the capacity of spleen cells from mice rejecting hybrid cells to stimulate the release of /sup 51/Cr from labeled ASL-1 cells was investigated. Cells from the spleens of mice rejecting ASL-1 x LM(TK)/sup -/ cells stimulated the release of /sup 51/Crmore » from labeled ASL-1 cells, but not from Ehrlich ascites or P815 cells. Cells from mice injected with mitomycin-C-treated ASL-1 cells led to the release of /sup 51/Cr from labeled ASL-1 cells as well, but the extent of /sup 51/Cr release was approximately one-third as occurred in the presence of cells from hybrid cell-injected mice. Cells from noninjected mice or from mice injected with LM(TK)/sup -/ cells failed to lead to the specific release of /sup 51/Cr from ASL-1 cells. The presence of unlabeled ASL-1 cells, but not Ehrlich ascites cells, competitively inhibited the spleen cell-stimulated release of /sup 51/Cr from labeled ASL-1 cells. Sera from A/J mice injected with mitomycin-C-treated ASL-1 cells contained antibodies specific for the tumor-associated antigen of ASL-1 cells.« less
  • The cellular basis of immunity to sporozoites was investigated by examining the effect of immunization of T and B cell-deficient C57BL/6N x BALB/c AnN F/sub 1/ (BLCF/sub 1/) mice compared to immunocompetent controls. Immunization of T cell-deficient (ATX-BM-ATS) BLCF/sub 1/ mice with x-irradiated sporozoites did not result in the generation of protective immunity. The same immunization protocols protected all immunocompetent controls. In contrast, B cell-deficient (..mu..-suppressed) BLCF/sub 1/ mice were protected by immunization in the majority of cases. The absence of detectable serum circumsporozoite precipitins or sporozoite neutralizing activity in the ..mu..-suppressed mice that resisted a sporozoite challenge suggests amore » minor role for these humoral factors in protection. These data demonstrate a preeminent role for T cells in the induction of protective immunity in BLCF/sub 1/ mice against a P. berghei sporozoite infection.« less
  • No abstract prepared.