Sugar-induced potential difference and short circuit current in bullfrog small intestine: effect of UO/sub 2//sup 2 +/ (39316)

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-152-39316· OSTI ID:7086600

Armstrong, W M; Arbelaez, J D

UO/sub 2//sup 2 +/ 1.3 mM added as UO/sub 2/(NO/sub 3/)/sub 2/ to the mucosal solution consistently inhibited the transmural potential difference (P,D) and short circuit current (I/sub sc/) evoked by 11 mM glucose and 35 mM 3-O-methyl glucose across isolated strips of bullfrog small intestine bathed by symmetrical Ringer solutions in which SO/sub 4//sup 2 -/ was the major anion. The average degree of inhibition in the presence of glucose was 42 +- 7 (SEM) percent. P.D. and I/sub sc/ in the absence of transported solutes were not significantly altered by mucosal UO/sub 2//sup 2 +/ at this concentration. Increasing the mucosal UO/sub 2//sup 2 +/ concentration to 2.6 mM did not significantly increase its inhibitory action on glucose-evoked P.D. and I/sub sc/. Further increasing the UO/sub 2//sup 2 +/ concentration to 13 mM completely inhibited glucose-induced P.D. and I/sub sc/ but also markedly reduced these parameters in the absence of glucose. Serosal UO/sub 2//sup 2 +/ (1.3 mM) had no effect on the P.D. and I/sub sc/ evoked by glucose and 3-O-methyl glucose. It is suggested that the inhibitory action of UO/sub 2//sup 2 +/ involves binding of this ion to anionic sites located in the apical membrane of the absorptive cells. Mucosal or serosal UO/sub 2//sup 2 +/ (1.3 mM) had no effect on the P.D. and I/sub sc/ elicited by 20 mM valine, indicating that under the conditions of these experiments UO/sub 2//sup 2 +/ selectively inhibits sugar-induced P.D. and I/sub sc/ and, by implication, mucosal sugar uptake.

• Research Organization: Indiana Univ., Indianapolis
• OSTI ID: 7086600
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 152; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

550500 -- Metabolism
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE COMPOUNDS
ALDEHYDES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
ANIONS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHARGED PARTICLES
CURRENTS
DIGESTIVE SYSTEM
FROGS
GASTROINTESTINAL TRACT
GLUCOSE
HEXOSES
INHIBITION
INTESTINES
IONS
MEMBRANES
MONOSACCHARIDES
MUCOUS MEMBRANES
NITRATES
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
QUANTITY RATIO
SACCHARIDES
SEROUS MEMBRANES
SMALL INTESTINE
SULFATES
SULFUR COMPOUNDS
UPTAKE
URANIUM COMPOUNDS
URANIUM OXIDES
VALINE
VERTEBRATES