Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.6.10.3559· OSTI ID:7083537

Valerie, K; Fronko, G; Henderson, E E; de Riel, J K

A plasmid containing the denV gene from bacteriophage T4, under the control of the yeast alcohol dehydrogenase I (ADC1) promoter, conferred a substantial increase in UV resistance in the UV-sensitive Saccharomyces cerevisiae mutants rad1-2 and rad3-2. The UV resistance of the denV+ yeast cells was cell cycle dependent and correlated well with the level of the denV gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-DNA glycosylase activity.

• Research Organization: Temple Univ. School of Medicine, Philadelphia, PA
• OSTI ID: 7083537
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 10; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOL DEHYDROGENASE
BACTERIA
BACTERIOPHAGES
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
FUNGI
GENES
HEMIACETAL DEHYDROGENASES
MICROORGANISMS
MUTANTS
OXIDOREDUCTASES
PARASITES
PLANTS
PLASMIDS
RADIATIONS
RADIOSENSITIVITY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
ULTRAVIOLET RADIATION
VIRUSES
YEASTS