Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Expression of the bacteriophage T4 denV structural gene in Escherichia coli

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:7083616
; ; ;

The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.

Research Organization:
Vanderbilt Univ. School of Medicine, Nashville, TN
OSTI ID:
7083616
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 2; ISSN JOBAA
Country of Publication:
United States
Language:
English

Similar Records

Expression of a cloned denV gene of bacteriophage T4 in Escherichia coli
Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5016934
Cloning, expression and stable maintenance of a bacteriophage DNA repair gene in E. coli
Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6905843
Site-directed mutagenesis of the T4 endonuclease V gene: role of lysine-130
Journal Article · Mon Mar 21 23:00:00 EST 1988 · Biochemistry; (United States) · OSTI ID:6840282

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
MICROORGANISMS
PARASITES
PHENOTYPE
PLASMIDS
RADIATIONS
RADIOSENSITIVITY
ULTRAVIOLET RADIATION
VIRUSES