DENV gene of bacteriophage T4 codes for both pyrimidine dimer-DNA glycosylase and apyrimidinic endonuclease activities

Journal Article · Thu Oct 01 04:00:00 EDT 1981 · J. Virol.; (United States)

OSTI ID:5927276

McMillan, S ^[1]; Edenberg, H J; Radany, E H; Friedberg, R C; Friedberg, E C

---

1. Stanford Univ., CA

Recent studies have shown that purified preparations of phage T4 UV DNA-incising activity (T4 UV endonuclease or endonuclease V of phase T4) contain a pyrimidine dimer-DNA glycosylase activity that catalyzes hydrolysis of the 5' glycosyl bond of dimerized pyrimidines in UV-irradiated DNA. Such enzyme preparations have also been shown to catalyze the hydrolysis of phosphodiester bonds in UV-irradiated DNA at a neutral pH, presumably reflecting the action of an apurinic/apyrimidinic endonuclease at the apyrimidinic sites created by the pyrimidine dimer-DNA glycosylase. In this study we found that preparations of T4 UV DNA-incising activity contained apurinic/apyrimidinic endonuclease activity that nicked depurinated form I simian virus 40 DNA. Apurinic/apyrimidinic endonuclease activity was also found in extracts of Escherichia coli infected with T4 denV/sup +/ phage. Extracts of cells infected with T4 denV mutants contained significantly lower levels of apurinic/apyrimidinic endonuclease activity; these levels were no greater than the levels present in extracts of uninfected cells. Furthermore, the addition of DNA containing UV-irradiated DNA and T4 enzyme resulted in competition for pyrimidine dimer-DNA glycosylase activity against the UV-irradiated DNA. On the basis of these results, we concluded that apurinic/apyrimidinic endonuclease activity is encoded by the denV gene of phage T4, the same gene that codes for pyrimidine dimer-DNA glycosylase activity.

• OSTI ID: 5927276
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 40:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOCHEMISTRY
CELL CULTURES
CHEMISTRY
CODONS
DATA
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
EXPERIMENTAL DATA
GENES
INFORMATION
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
RADIATIONS
RADIOSENSITIVITY
ULTRAVIOLET RADIATION
VIRUSES