Studies on the 1. cap alpha. ,25-dihydroxycholecalciferol-like activity in a calcinagenic plant, Cestrum diurnum, in the chick

Journal Article · Thu Apr 01 04:00:00 EST 1976 · J. Nutr.; (United States)

OSTI ID:7083303

Wasserman, R H ^[1]; Corradino, R A; Krook, L; Hughes, M R; Haussler, M R

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1. Cornell Univ., Ithaca, NY

Cestrum diurnum (day-blooming jessamine) has been proposed to cause calcinosis in horses and cattle in Florida. The present studies investigated some physiological properties of the plant, using the chick as the experimental animal. The inclusion of dried leaf powder in a rachitogenic diet restored intestinal calcium-binding protein synthesis (CaBP) and increased calcium absorption in the cholecalciferol-deficient chick. The estimated level of cholecalciferol-equivalents in the dried leaf was about 30,000 to 35,000 IU/kg. Most of the activity was extractable with methanol : chloroform (2:1), indicating that the major cholecalciferol-like component in C. diurnum was different from the water soluble factor(s) in Solanum malacoxylon. The time course of effect of C. diurnum extract in rachitic chicks was similar to that of 1,25-dihydroxycholecalciferol but the former had a longer lag time. The strontium fed chick, in which the kidney 25-hydroxycholecaliciferol-1..cap alpha..-hydroxylase is inhibited, responded to C. diurnum extract, confirming the 1..cap alpha..,25-dihydroxycholecalciferol-like character of the Cestrum factor. The extract also appeared to interact with the intestinal 1..cap alpha..,25-dihydroxycholecalciferol cytosol receptor although this observation is preliminary. These findings indicate that the 1..cap alpha..,25-dihydroxycholecalciferol-like principle in C. diurnum may cause excessive calcium and phosphate absorption leading to calcinosis.

• OSTI ID: 7083303
• Journal Information: J. Nutr.; (United States), Journal Name: J. Nutr.; (United States) Vol. 106:4; ISSN JONUA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMALS
BIOLOGICAL EFFECTS
BIOMASS
BIRDS
CALCIUM
CHICKENS
CHOLECALCIFEROL
DEPOSITION
ELEMENTS
ENERGY SOURCES
FOWL
INTESTINAL ABSORPTION
METABOLISM
METALS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PLANTS
RENEWABLE ENERGY SOURCES
STRONTIUM
UPTAKE
VERTEBRATES
VITAMIN D
VITAMINS