Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin: structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones
The authors have elucidated the structures of the anionic asparagine-linked oligosaccharides present on the glycoprotein hormones lutropin (luteinizing hormone), follitropin (follicle-stimulating hormone), and thyrotropin (thyroid-stimulating hormone). Purified hormones, isolated from bovine, ovine, and human pituitaries, were digested with N-glycanase, and the released oligosaccharides were reduced with NaB(/sup 3/H)/sub 4/. The /sup 3/H-labeled oligosaccharides from each hormone were then fractionated by anion-exchange high performance liquid chromatography (HPLC) into populations differing in the number of sulfate and/or sialic acid moieties. The sulfated, sialylated, and sulfated/sialylated structures, which together comprised 67-90% of the asparagine-linked oligosaccharides on the pituitary glycoprotein hormones, were highly heterogeneous and displayed hormone- as well as animal species-specific features. A previously uncharacterized dibranched oligosaccharide, bearing one residue each of sulfate and sialic acid, was found on all of the hormones except bovine lutropin. In this study, they describe the purification and detailed structural characterizations of the sulfated, sialylated, and sulfated/sialylated oligosaccharides found on lutropin, follitropin, and thyrotropin from several animal species.
- Research Organization:
- Washington Univ. Medical School, St. Louis, MO (USA)
- OSTI ID:
- 7075898
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:1
- Country of Publication:
- United States
- Language:
- English
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OLIGOSACCHARIDES
BIOCHEMISTRY
PITUITARY HORMONES
MOLECULAR STRUCTURE
TRITIUM COMPOUNDS
ASPARAGINE
CATTLE
FSH
GLUCOPROTEINS
ION EXCHANGE CHROMATOGRAPHY
LH
MAN
PITUITARY GLAND
TSH
AMIDES
AMINO ACIDS
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
CHROMATOGRAPHY
DOMESTIC ANIMALS
ENDOCRINE GLANDS
GLANDS
GLYCOPROTEINS
GONADOTROPINS
HORMONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PRIMATES
PROTEINS
RUMINANTS
SACCHARIDES
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques