Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin: distributions of sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones

Journal Article · Tue Jan 05 04:00:00 EST 1988 · J. Biol. Chem.; (United States)

OSTI ID:7075879

Green, E D; Baenziger, J U

The asparagine-linked oligosaccharides on the pituitary glycoprotein hormones lutropin (LH), follitropin (FSH), and thyrotropin (TSH) consist of a heterogeneous array of neutral, sulfated, sialylated, and sulfated/sialylated structures. In this study, the authors determined the relative quantities of the various asparagine-linked oligosaccharides on LH, FSH, and TSH from these three animal species. The proportions of sulfated versus sialylated oligosaccharides varied markedly among the different hormones. Both hormone- and animal species-specific differences in the types and distributions of sulfated, sialylated, and sulfated/sialylated structures were evident. In particular, LH and FSH, which are synthesized in the same pituitary cell and bear ..cap alpha..-subunits with the identical amino acid sequence, contained significantly different distributions of sulfated and sialylated oligosaccharides. For all three animal species, the ratio of sialylated to sulfated oligosaccharides differed by >10-fold for LH and FSH, with sulfated structures dominating on LH and sialylated structures on FSH. Sialylated oligosaccharides were also heterogeneous with respect to sialic acid linkage (..cap alpha..2,3 versus ..cap alpha..2,6). The differences in oligosaccharide structures among the various pituitary glycoprotein hormones as well as among the various glycosylation sites within a single hormone support the hypothesis that glycosylation may serve important functional roles in the expression and/or regulation of hormone bioactivity.

• Research Organization: Washington Univ. Medical School, St. Louis, MO (USA)
• OSTI ID: 7075879
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:1; ISSN JBCHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
ANIMALS
ASPARAGINE
BIOCHEMISTRY
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CATTLE
CHEMISTRY
CHROMATOGRAPHY
DOMESTIC ANIMALS
ENDOCRINE GLANDS
FSH
GLANDS
GLUCOPROTEINS
GLYCOPROTEINS
GONADOTROPINS
HORMONES
ION EXCHANGE CHROMATOGRAPHY
LABELLED COMPOUNDS
LH
MAMMALS
MAN
MOLECULAR STRUCTURE
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
PRIMATES
PROTEINS
PURIFICATION
RUMINANTS
SACCHARIDES
SEPARATION PROCESSES
TRITIUM COMPOUNDS
TSH
VERTEBRATES