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Biosynthesis of lutropin in ovine pituitary slices: incorporation of (/sup 35/S)sulfate in carbohydrate units

Journal Article · · Arch. Biochem. Biophys.; (United States)
;
Sulfate incorporation into carbohydrate of lutropin (LH) has been studied in sheep pituitary slices using H/sub 2/(/sup 35/)SO/sub 4/. Labeled ovine LH was purified to homogeneity by Sephadex G-100 and carboxymethyl-Sephadex chromatography from both the incubation medium and tissue extract. Autoradiography of the gel showed only two protein bands which comigrated with the alpha and beta subunits of ovine LH in both the purified ovine LH and the immunoprecipitate obtained with LH-specific rabbit antiserum. Furthermore, (/sup 35/S)sulfate was also incorporated into several other proteins in addition to LH. The location of /sup 35/SO/sub 2-(4)/ in the oligosaccharides of ovine LH was evidenced by its presence in the glycopeptides obtained by exhaustive Pronase digestion. The location and the point of attachment of sulfate in the carbohydrate unit were established by the isolation of 4-O-(/sup 35/S)sulfo-N-acetylhexosaminyl-glycerols and 4-O-(/sup 35/S) sulfo-N-acetylglucosaminitol from the Smith degradation products and by the release of /sup 35/SO/sub 2-(4)/ by chondro-4-sulfatase. Thus, the present line of experimentation indicates the presence of sulfate on both the terminal N-acetylglucosamine and N-acetylgalactosamine in the oligosaccharide chains of the labeled ovine LH.
Research Organization:
Department of Biological Sciences, Division of Cell and Molecular Biology, State University of New York, Buffalo
OSTI ID:
6191934
Journal Information:
Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 220:2; ISSN ABBIA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BINDING ENERGY
BIOSYNTHESIS
BODY
CARBOHYDRATES
CHEMICAL PREPARATION
CHEMICAL PROPERTIES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTROPHORESIS
ENDOCRINE GLANDS
ENERGY
EVEN-ODD NUCLEI
GLANDS
GONADOTROPINS
HORMONES
ISOTOPES
LABELLED COMPOUNDS
LH
LIGHT NUCLEI
MAMMALS
MOLECULAR BIOLOGY
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
SHEEP
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES