Radiosensitizing and toxic effects of the 2-nitroimidazole Ro-07-0582 in different phases of the cell cycle of extremely hypoxic human cells in vitro

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574582· OSTI ID:7075815

Petterson, E O

The radiosensitizing effect of 5 and 30 mM of Ro-07-0582 (misonidazole) has been studied at different stages of the cell cycle of mitotically selected NHIK 3025 cells under aerobic and extremely hypoxic conditions. For cells irradiated under aerobic conditions no sensitizing effect was seen at any stage of the cell cycle. For cells irradiated under extremely hypoxic conditions there was a lower sensitizing effect in mid-G1 than in mid-S for low radiation doses (in the initial region of the dose-response curves). For high radiation doses, however, no significant difference in sensitizing effect on cells in mid-G1 and in mid-S was seen. For cells in mid-G1 the sensitizing effect increased with increasing radiation dose. The toxic effect of 30 mM Ro-07-0582 as measured by loss of reproductive capacity was studied at room temperature for contact times up to 6 hours under aerobic conditions and 3 hours under extremely hypoxic conditions. While no effect was seen under aerobic conditions there was a toxic effect for contact intervals above 1 hour under extremely hypoxic conditions. Cells in S were more sensitive to the toxic effect of Ro-07-0582 than cells in G1. Implications for clinical use are discussed.

• Research Organization: Norwegian Radium Hospital, Oslo
• OSTI ID: 7075815
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 73:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANOXIA
AZOLES
CELL CULTURES
CELL CYCLE
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IONIZING RADIATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
TOXICITY
X RADIATION