Desialation of transferrin by rat liver endothelium

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · J. Clin. Invest.; (United States)

DOI:https://doi.org/10.1172/JCI113625· OSTI ID:7074916

Irie, S; Kishimoto, T; Tavassoli, M

To examine the role of liver endothelium in desialation of transferrin (TF), pulse-chase studies were done by incubation of either /sup 3/H (sialic acid labeled)-, or /sup 125/I, or /sup 59/Fe (protein core labeled)-TF with fractionated liver endothelium. While /sup 125/I or /sup 59/Fe labels were externalized after initial binding and internalization, a large proportion of 3H label was internalized and remained within the cell. When the supernatant of these experiments was studied by isoelectricfocusing and Ricinus communis agglutinin (RCA120) affinity chromatography, generation of asialotransferrin was noted by both techniques. Incubation of liver endothelium with double-labeled TF (sialic acids with /sup 3/H and protein core with /sup 125/I or /sup 59/Fe) led initially to a concordant uptake of the two labels, which were then dissociated and /sup 3/H was retained by the cell. These findings indicate desialation of TF by liver endothelium. The significance of these findings in the pathogenesis of hepatic siderosis is discussed.

• Research Organization: Veterans Administration Medical Center, Jackson, MS (USA)
• OSTI ID: 7074916
• Journal Information: J. Clin. Invest.; (United States), Journal Name: J. Clin. Invest.; (United States) Vol. 82:2; ISSN JCINA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LECTINS
LIVER
MAMMALS
METABOLIC DISEASES
METABOLISM
METALLOPROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SIALIC ACID
TISSUES
TRACER TECHNIQUES
TRANSFERRIN
TRITIUM COMPOUNDS
VERTEBRATES