Double labeling of transferrin: tritium labeling of sialic acid and /sup 125/I or /sup 59/Fe labeling of the protein moiety
Journal Article
·
· Anal. Biochem.; (United States)
A method is described in which the glycoprotein transferrin was double labeled. Its sialic acid residues were labeled with /sup 3/H through a consecutive oxidation-reduction technique utilizing tritiated NaBH/sub 4/. Its protein moiety was labeled with either /sup 125/I or /sup 59/Fe. Incubation of this double-labeled molecule at 4/sup 0/C with K562 cells gave overlapping curves, indicating identical patterns of binding for all labels. At 37/sup 0/C, /sup 3/H and /sup 125/I demonstrated identical patterns while /sup 59/Fe was cummulatively retained. This method can be used to follow the fate of other glycoproteins and their possible desialation in vivo.
- Research Organization:
- Univ. of Mississippi Medical Center, Jackson
- OSTI ID:
- 5496719
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 153:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:5226566
Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
GLUCOPROTEINS
IN VIVO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON 59
IRON ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
METALLOPROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SIALIC ACID
TRANSFERRIN
TRITIUM COMPOUNDS
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
GLUCOPROTEINS
IN VIVO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRON 59
IRON ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
METALLOPROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SIALIC ACID
TRANSFERRIN
TRITIUM COMPOUNDS