Increased glucose dependence in resting, iron-deficient rats

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · American Journal of Physiology; (USA)

OSTI ID:7068494

Brooks, G A; Henderson, S A; Dallman, P R ^[1]

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1. Univ. of California, Berkeley (USA) Univ. of California, San Francisco (USA)

Rates of blood glucose and lactate turnover were assessed in resting iron-deficient and iron-sufficient (control) rats to test the hypothesis that dependence on glucose metabolism is increased in iron deficiency. Male Sprague-Dawley rats, 21 days old, were fed a diet containing either 6 mg iron/kg feed (iron-deficient group) or 50 mg iron/kg feed (iron-sufficient group) for 3-4 wk. The iron-deficient group became anemic, with hemoglobin levels of 6.4 {plus minus} 0.2 compared with 13.8 {plus minus} 0.3 g/dl for controls. Rats received a 90-min primed continuous infusion of D-(6-{sup 3}H)glucose and sodium L-(U-{sup 14}C)lactate via a jugular catheter. Serial samples were taken from a carotid catheter for concentration and specific activity determinations. Iron-deficient rats had significantly higher blood glucose and lactate concentrations than controls. The iron-deficient group had a significantly higher glucose turnover rate than the control group. Significantly more metabolite recycling in iron-deficient rats was indicated by greater incorporation of {sup 14}C into blood glucose. Assuming a carbon crossover correction factor of 2, half of blood glucose arose from lactate in deficient animals. By comparison, only 25% of glucose arose from lactate in controls. Lack of a difference in lactate turnover rates between deficient rats and controls was attributed to {sup 14}C recycling. The results indicate a greater dependence on glucose metabolism in iron-deficient rats.

• OSTI ID: 7068494
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:4; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALDEHYDES
ANEMIAS
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
DIET
DISEASES
GLUCOSE
HEMIC DISEASES
HEXOSES
HYDROGEN COMPOUNDS
HYDROXY ACIDS
IRON COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LACTIC ACID
METABOLISM
MONOSACCHARIDES
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
SACCHARIDES
SYMPTOMS
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS