Glucose kinetics at rest and during exercise in gluconeogenesis-inhibited rats
To evaluate the role played by gluconeogenesis in blood glucose homeostasis, untrained and trained rats were injected with mercaptopicolinic acid (MPA), a known inhibitor of the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase. Glucose turnover, recycling and oxidation rates were assessed by primed-continuous infusion of (U-{sup 14}C)- and (6-{sup 3}H) glucose at rest and during submaximal exercise at 13.4 m/min on level grade. When compared to the untrained sham-injected animals, the untrained MPA-treated animals had 22% lower and 44% higher resting blood glucose and lactate concentrations, respectively. Resting glucose turnover, calculated from (6-{sup 3}H)glucose, was 32% lower in the MPA-treated animals than in the sham-injected animals. During exercise, turnover increased in the sham-injected animals but remained unchanged in the MPA-treated animals. MPA-treated animals had no glucose recycling at rest or during exercise. Exercise further decreased blood glucose concentration and increased blood lactate concentration in the MPA-treated animals, but MPA treatment did not change the exercise-induced increases in glucose oxidation rate, % total VCO{sub 2} arising from glucose oxidation and metabolic clearance rate of glucose.
- Research Organization:
- California Univ., Berkeley, CA (USA)
- OSTI ID:
- 6810176
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
CARBOHYDRATES
ENZYME INHIBITORS
EXERCISE
GLUCOSE
HEXOSES
HOMEOSTASIS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
RATS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES