Effects of ozonation on mutagenic activity of polycyclic aromatic hydrocarbons

Journal Article · Mon Jul 01 04:00:00 EDT 1991 · Bulletin of Environmental Contamination and Toxicology; (United States)

DOI:https://doi.org/10.1007/BF01689445· OSTI ID:7066269

Fouillet, B; Chambon, P; Chambon, R ^[1]; Castegnaro, M ^[2]; Weill, N ^[3]

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1. Faculty of Pharmaceutical Sciences, Lyon (France)
2. International Agency for Research on Cancer, Lyon (France)
3. Hazleton, L'Arbresle (France)

In this study, four polycyclic aromatic hydrocarbons were tested. Benzo(a)pyrene (B(a)P), Chrysene (CH), 7,12-dimethylbenzo(a)-anthracene (DMBA) and 3-methylcholanthrene (MCA) in hexane were treated with ozone to determine the effectiveness of degradation and to evaluate the genetic properties of ozone byproducts. Two types of ozonation were carried out: partial ozonation and total ozonation. The disappearance of parent compounds and the appearance of ozone byproducts were measured by high performance liquid chromatography (HPLC) coupled with spectrofluorimetry and U.V. spectrophotometry. Plate incorporation mutagenicity assay, using a Salmonella typhimurium strain, was used to test the ozone byproducts with and without metabolic activation.

• OSTI ID: 7066269
• Journal Information: Bulletin of Environmental Contamination and Toxicology; (United States), Journal Name: Bulletin of Environmental Contamination and Toxicology; (United States) Vol. 47:1; ISSN 0007-4861; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BENZANTHRACENE
BENZOPYRENE
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
CHRYSENE
CONDENSED AROMATICS
DECOMPOSITION
HYDROCARBONS
MICROORGANISMS
MUTAGEN SCREENING
ORGANIC COMPOUNDS
OZONE
POLYCYCLIC AROMATIC HYDROCARBONS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING