Engraftment of DLA-nonidentical unrelated canine marrow after high-dose fractionated total body irradiation
- Fred Hutchinson Cancer Research Center, Seattle, WA
Marrow transplants were carried out between unrelated DLA-nonidentical dogs. Recipients were conditioned for transplantation by total body irradiation (TBI) given either as a single dose of 9 Gy (900 rad) or fractionated in three increments of 6 Gy (600 rad) each at intervals of 48 hr. All recipients received marrow, less than or equal to to 4 X 10/sup 8/ cells/kg, and no buffy coat cells. No immunosuppression was given after grafting. All 10 dogs given single-dose total body irradiation failed to show engraftment and died with marrow aplasia and infectious complications (median survival 12 days). In contrast, all 10 dogs given fractionated TBI had sustained engraftment and died with graft-versus-host disease (GVHD) and infectious complications (median survival 12.5 days). None of the dogs died from radiation-induced gastroenteritis.In conclusion, resistance to DLA-nonidentical unrelated marrow grafts can be abrogated by high-dose TBI. This technique may allow hemopoietic engraftment even after in vitro manipulation of the marrow such as lymphocyte depletion by cell separation or treatment with anti-T cell antisera.
- OSTI ID:
- 7066015
- Journal Information:
- Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 33:4; ISSN TRPLA
- Country of Publication:
- United States
- Language:
- English
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560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD COUNT
BODY
BONE MARROW
COBALT 60
COBALT ISOTOPES
DISEASES
DOGS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GAMMA RADIATION
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IMMUNITY
IMMUNOSUPPRESSION
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
TISSUES
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES