Engraftment of DLA-nonidentical unrelated canine marrow after high-dose fractionated total body irradiation
Marrow transplants were carried out between unrelated DLA-nonidentical dogs. Recipients were conditioned for transplantation by total body irradiation (TBI) given eigher as a single dose of 9 Gy (900 rad) or fractionated in three increments of 6 Gy (600 rad) each at intervals of 48 hr. All recipients received marrow, less than or equal to 4 x 10(8) cells/kg, and no buffy coat cells. No immunosuppression was given after grafting. All 10 dogs given single dose total body irradiation failed to show engraftment and died with marrow aplasia and infectious complications (median survival 12 days). In contrast, all 10 dogs given fractionated TBI had sustained engraftment and died with graft-versus-host disease (GVHD) and infectious complications (median survival 12.5 days). None of the dogs died from radiation-induced gastroenteritis. In conclusion, resistance to DLA-nonidentical unrelated marrow grafts can be abrogated by high-dose TBI. This technique may allow hemopoietic engraftment even after i vitro manipulation of the marrow such as lymphocyte depletion by cell separation or treatment with anti-T cell antisera.
- Research Organization:
- Fred Hutchinson Cancer Research Center, Settle, WA
- OSTI ID:
- 6928797
- Journal Information:
- Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 33:4; ISSN TRPLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551000 -- Physiological Systems
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
DOGS
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
IMMUNOSUPPRESSION
IRRADIATION
MAMMALS
ORGANS
RADIATION DOSES
RADIATION EFFECTS
TISSUES
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION