Marrow grafts between phenotypically DLA-identical and haploidentical unrelated dogs: additional antigens controlling engraftment are not detected by cell-mediated lympholysis
- Fred Hutchinson Cancer Research Center, Seattle, WA
Bone marrow transplants with low marrow cell doses (less than or equal to4 X 10/sup 8/ cells/kg) from unrelated donors were carried out in 16 dogs conditioned with 9 Gy (900 rad) of total body irradiation. No immunosuppression was given after grafting. Eleven donor-recipient pairs were phenotypically identical (group 1) for the known antigens of the canine major histocompatibility complex (DLA) and in five the donor was homozygous and the recipient heterozygous for DLA (group 2), as determined by serological histocompatibility typing and mixed leukocyte cultures including homozygous cell typing. In addition, lymphocytes from donors and recipients in group 1 were mutually nonreactive in cell-mediated lympholysis; lymphocytes from recipients in group 2 were not cytotoxic against donor cells. Eight dogs rejected their grafts and eight showed sustained engraftment; of these, four died from graft-versus-host disease. The incidence of rejection was higher than in DLA-identical littermates but lower than in DLA-nonidentical unrelated or littermate dogs. These results indicate that antigens different from the recognized alleles at DLA are involved in the control of engraftment. These antigens most likely represent the expression of unrecognized differences within DLA or are coded for by a locus different from but linked to DLA-A, B, C or D; they are not recognized in the cell-mediated lympholysis assay.
- OSTI ID:
- 6722147
- Journal Information:
- Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 33:1; ISSN TRPLA
- Country of Publication:
- United States
- Language:
- English
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560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DOGS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GAMMA RADIATION
GRAFT-HOST REACTION
GRAFTS
HEMATOPOIETIC SYSTEM
IMMUNOLOGY
IMMUNOSUPPRESSION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYSIS
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANS
PHENOTYPE
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES