Subcellular distribution and chemical form of cadmium in bean plants

Journal Article · Sat Mar 01 04:00:00 EST 1980 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.65.3.480· OSTI ID:7056096

Weigel, H J; Jaeger, H J

The subcellular distribution and chemical form of Cd in bean plants grown in nutrient solutions containing Cd were investigated. Cd was accumulated mainly in roots and to a minor extent in leaves. Subcellular fractionation of Cd-containing tissues (pH 7.5) showed that more than 70% of the element was localized in the cytoplasmic fraction in roots as well as in leaves. Little Cd (8 to 14%) was bound either to the cell wall fraction or to the organelles. Gel filtration of the soluble fraction showed Cd to be associated mainly with 5000 to 10,000 molecular weight components in roots, and 700 to 5000 molecular weight components in leaves. Small amounts of Cd were found in the high molecular weight proteins (molecular weight 150,000). Only traces of Cd could be detected as a free ion. Chemical characterization of the low molecular weight components resulted in the identification of nine amino acids which were identical in roots and leaves. Cd in bean plants is assumed to be bound to peptides and/or proteins of low molecular weight.

• Research Organization: Justus Liebig-Universitaet, Giessen, Germany
• OSTI ID: 7056096
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 65:3; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGY
BOTANY
CADMIUM
CELL CONSTITUENTS
CULTURE MEDIA
CYTOPLASM
ELEMENTS
KINETICS
LEAVES
LEGUMINOSAE
METABOLISM
METALS
MOLECULAR WEIGHT
NUTRIENTS
PHASEOLUS
PLANT CELLS
PLANT TISSUES
PLANTS
REACTION KINETICS
ROOTS
SENSITIVITY
TOXICITY