Subcellular site and nature of intracellular cadmium in plants
The mechanisms underlying heavy metal accumulation, toxicity and tolerance in higher plants are poorly understood. Since subcellular processes are undoubtedly involved in all these phenomena, it is of interest to study the extent of, subcellular site of and nature of intracellularly accumulated cadmium in higher plants. Whole plants supplied /sup 109/CdCl/sub 2/ or /sup 112/CdSO/sub 4/ accumulated Cd into roots and aerial tissues. Preparation of protoplasts from aerial tissue followed by subcellular fractionation of the protoplasts to obtain intact vacuoles, chloroplasts and cytosol revealed the presence of Cd in the cytosol but not in vacuoles or chloroplasts. Particulate materials containing other cell components were also labeled. Of the /sup 109/Cd supplied to plants, 2 to 10% was recovered in both cytosol preparations and in particulate materials. Cytosol contained proteinaceous--Cd complexes, free metal and low molecular weight Cd complexes. Labeling of protoplasts gave similar results. No evidence was obtained for the production of volatile Cd complexes in tobacco.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- EY-76-C-02-0016
- OSTI ID:
- 6144936
- Report Number(s):
- BNL--26259; CONF-790673--1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
BETA DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
CADMIUM
CADMIUM 109
CADMIUM ISOTOPES
COLLOIDS
DEPOSITION
DISPERSIONS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
METABOLISM
METALS
NICOTIANA
NUCLEI
PLANTS
RADIOISOTOPES
SOLS
TOLERANCE
TOXICITY
YEARS LIVING RADIOISOTOPES