Subcellular localization of Cd and Cd-binding peptides in tobacco leaves

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6936376

Vogeli-Lange, R; Wagner, G J ^[1]

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1. Univ. of Kentucky, Lexington (USA)

Cd-binding peptides (CdBP's) having the general structure {gamma}-(Glu-Cys){sub n}-Gly are inducible by and have high affinity for Cd. If these peptides are involved in Cd detoxification by chelation, both metal and ligand must be localized in the same cellular compartment. To address this question, we studied the vacuolar/extravacuolar distribution of Cd and CdBP's in leaves of hydroponically grown tobacco seedlings. CdBP's were induced upon addition of 20 {mu}M CdCl{sub 2} (non-phytotoxic level) to the nutrient solution. Amino acid analysis indicated that the main components were {gamma}-(Glu-Cys){sub 3}-Gly and {gamma}-(Glu-Cys){sub 4}-Gly. Purified vacuoles isolated from protoplasts of Cd treated leaves contained most of the total CdBP's and Cd found in protoplasts (104% {plus minus}8 and 110% {plus minus}8, respectively). The probability that CdBP's are synthesized extravacuolarly and their predominant location in the vacuole suggest that these molecules may be involved in translocation of Cd to the vacuole.

• OSTI ID: 6936376
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
CADMIUM
CADMIUM CHLORIDES
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHLORIDES
CHLORINE COMPOUNDS
DETOXIFICATION
DISTRIBUTION
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
LEAVES
METALS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
SEEDLINGS
SUBCELLULAR DISTRIBUTION
TOBACCO
TRANSLOCATION