Phytohemagglutinin induced proliferation by aged lymphocytes: reduced expression of high affinity interluekin-2 receptors and interleukin-2 secretion
Journal Article
·
· Life Sci.; (United States)
Human lymphocytes from elderly and young donors were cultured with phytohemagglutinin. Cultures from two groups of aged donors, recruited respectively from the authors ambulatory clinic and a nursing home, incorporated less tritiated thymidine (/sup 3/H-TdR) and secreted less interleukin-2 than did young donors. Furthermore, as determined for the first time by a radioligand binding receptor assay, the aged lymphoblasts possessed significantly fewer high affinity IL-2 receptors per cell. Despite a decrease in the number of high affinity receptor cells the dissociation constant (Kd) was comparable for the three groups. It was also shown that the amounts of soluble IL-2 receptors that were released into the supernatants by mitogen stimulated cells did not differ for the aged and young donors. These data suggest that defects in IL-2 production and high affinity IL-2 receptor generation may both be responsible for immune deficiency in the elderly.
- Research Organization:
- Univ. of Illinois, Chicago (USA)
- OSTI ID:
- 7055683
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 43:20; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AGE DEPENDENCE
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
HEMAGGLUTININS
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIORECEPTOR ASSAY
RECEPTORS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AGE DEPENDENCE
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
HEMAGGLUTININS
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIORECEPTOR ASSAY
RECEPTORS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES