Effect of radiation on the production of interleukins and T-lymphocyte activities

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · J. Natl. Cancer Inst.; (United States)

OSTI ID:5012567

Manori, I; Kushelevsky, A; Segal, S; Weinstein, Y

The effect of 0-400 rad /sup 60/Co gamma-ray doses on distinct steps in the process of murine T-cell activation by concanavalin A (Con A) was investigated. When C57BL/6 spleen cells were stimulated immediately after irradiation, production of interleukin-1 (IL-1) and interleukin-2 (IL-2) was not impaired. Concomitantly, the display of IL-2 receptors in Con A-induced reactivity to IL-2 was not affected. The proliferative response was markedly diminished by increasing doses of radiation. The effect of radiation was found to depend not only on the delivered dose but also on the time interval between irradiation and stimulation of the lymphoid cultures. When the mitogenic stimulus was delayed for 24 hours following irradiation, IL-1 production was not diminished, whereas IL-2 production was impaired by doses greater than 200 rad. The proliferative response was diminished to a markedly higher degree as compared to the degree it was diminished in cell cultures stimulated by Con A immediately after irradiation. IL-2 production and the proliferative response to Con A of irradiated cell suspensions, cultured without mitogen for 24 hours post irradiation, were also assessed after adjustment for cell death. In this case, an impairment in IL-2 production that was dose dependent was apparent, but still the levels of IL-2 secreted by 400-rad irradiated cells reached high levels. In contrast, the proliferative response to Con A could not be restored. When T-cell growth factor was added concomitantly with Con A to irradiated cell cultures, a radioprotective effect could be observed.

• OSTI ID: 5012567
• Journal Information: J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 6; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIVISION
CELL PROLIFERATION
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DOSES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HEMAGGLUTININS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MICE
MITOGENS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RECEPTORS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SYNTHESIS
VERTEBRATES