beta. -Adrenergic receptor-mediated suppression of interleukin 2 receptors in human lymphocytes

Journal Article · Sun Nov 15 04:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5301564

Feldman, R D; Hunninghake, G W; McArdle, W L

Adrenergic receptor agonists are know to attenuate the proliferative response of human lymphocytes after activation; however, their mechanism of action is unknown. Since expression of interleukin 2 (IL-2) receptors is a prerequisite for proliferation, the effect of ..beta..-adrenergic receptor agonists on lymphocyte IL-2 receptors was studied on both mitogen-stimulated lymphocytes and IL-2-dependent T lymphocyte cell lines. In both cell types the ..beta..-adrenergic receptor agonist isoproterenol blocked the expression of IL-2 receptors, as determined with the IL-2 receptor anti-TAC antibody. To determine the effect of ..beta..-adrenergic agonists on expression of the high affinity IL-2 receptors, (/sup 125/I)IL-2 binding studies were performed at concentrations selective for high affinity sites. No significant effect of ..beta..-adrenergic agonists on high affinity IL-2 receptor sites could be detected. The data demonstrate that ..beta..-adrenergic receptor agonists down-regulate IL-2 receptors primarily affecting low affinity sites.

• Research Organization: Univ. of Iowa College of Medicine, Iowa City
• OSTI ID: 5301564
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:10; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MITOGENS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
RIBOSIDES
SOMATIC CELLS
SYMPATHOMIMETICS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES