Studies of the stimulation and desensitization of beta adrenergic receptors in the human lymphocyte

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5148526

Borst, S E

Lymphocytes, were employed in radioligand binding studies of beta-adrenergic receptor density and affinity for agonists and in studies of isoproterenol stimulated adenylate cyclase activity. Studies of isoproterenol stimulation of adenylate cyclase activity demonstrated a role for extracellular calcium ions but not for extracellular magnesium ions in this process. Responses were diminished by chelation or by removal of extracellular calcium, as well as by lanthanum ion which competes with calcium for membrane binding sites. Initial studies using /sup 3/H-dihydroalprenolol (/sup 3/H-DHA) indicated that 40% of cell surface beta receptors are lost during exposure of lymphocytes to isoproterenol and that the remaining receptors have a reduced affinity for beta agonists. Results from studies with /sup 125/iodocyanopindolol (/sup 125/ICYP) are consistent with the view that beta receptors lost from the cell surface during isoproterenol treatment are present in a internal compartment of the cell. In mild asthmatic patients receiving a three week regimen of oral terbutaline a 40% reduction in the total receptor population was observed, suggesting that degradation of internalized receptors had occurred.

• Research Organization: California Univ., Los Angeles (USA)
• OSTI ID: 5148526
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ASTHMA
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPOUNDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHELATES
COMPLEXES
CONNECTIVE TISSUE CELLS
CYCLASES
DATA
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LANTHANUM COMPOUNDS
LEUKOCYTES
LIGANDS
LYASES
LYMPHOCYTES
MAGNESIUM COMPOUNDS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RARE EARTH COMPOUNDS
REACTION KINETICS
RECEPTORS
REMOVAL
RESPIRATORY SYSTEM DISEASES
SOMATIC CELLS
STIMULATION
SYMPATHOLYTICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS