Biochemical and metabolic response to nitrogen dioxide-induced endothelial injury. Research report, April 1983-January 1987

Technical Report · Mon Jun 01 04:00:00 EDT 1987

OSTI ID:7055652

Patel, J M; Block, E R

This study used tissue culture monolayers of porcine pulmonary artery and aortic endothelial cells to investigate the effects of 3 and 5-ppm nitrogen dioxide (NO/sub 2/) on cell membranes. Biophysical changes in the membrane preceded, by many hours, the metabolic uptake and biochemical and enzymatic changes in the cell. Membrane fluidity was found to be reduced in both hydrophobic and hydrophilic regions of the plasma membrane after three hours of exposure. This was accompanied by an increase in lipid content in the membrane. After 24 hours of exposure, NO(2-) induced injury was observed, with a decreased uptake of 5-hydroxytryptamine and an increase in lactate dehydrogenase release. Other effects observed include an increase in lipid peroxide formation and an increase in antioxidant enzyme activities. It was found that all abnormal biochemical and biophysical changes induced by NO/sub 2/ exposure were reversed 24 to 48 hours after exposure.

• Research Organization: Health Effects Inst., Cambridge, MA (USA)
• OSTI ID: 7055652
• Report Number(s): PB-88-234406/XAB; HEI/RR-87/09
• Country of Publication: United States
• Language: English

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500200 -- Environment
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54 ENVIRONMENTAL SCIENCES
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59 BASIC BIOLOGICAL SCIENCES
AIR POLLUTION
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
DOCUMENT TYPES
DOMESTIC ANIMALS
ENZYMES
EPITHELIUM
INJURIES
MAMMALS
METABOLISM
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANS
OXIDES
OXYGEN COMPOUNDS
POLLUTION
PROGRESS REPORT
SWINE
TISSUES
VERTEBRATES