Effect of plasma membrane fluidity on serotonin transport by endothelial cells

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6896925

Block, E R; Edwards, D ^[1]

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1. Univ. of Florida College of Medicine, Gainesville (USA)

To evaluate the effect of plasma membrane fluidity of lung endothelial cells on serotonin transport, porcine pulmonary artery endothelial cells were incubated for 3 h with either 0.1 mM cholesterol hemisuccinate, 0.1 mM cis-vaccenic acid, or vehicle (control), after which plasma membrane fluidity and serotinin transport were measured. Fluorescence spectroscopy was used to measure fluidity in the plasma membrane. Serotonin uptake was calculated from the disappearance of ({sup 14}C)-serotonin from the culture medium. Cholesterol decreased fluidity in the subpolar head group and central and midacyl side-chain regions of the plasma membrane and decreased serotonin transport, whereas cis-vaccenic acid increased fluidity in the central and midacyl side-chain regions of the plasma membrane and also increased serotonin transport. Cis-vaccenic acid had no effect of fluidity in the subpolar head group region of the plasma membrane. These results provide evidence that the physical state of the central and midacyl chains within the pulmonary artery endothelial cell plasma membrane lipid bilayer modulates transmembrane transport of serotonin by these cells.

• OSTI ID: 6896925
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKENES
AMINES
ANIMAL TISSUES
ANIMALS
ANISOTROPY
AROMATICS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BLOOD VESSELS
BODY
CARBON 14 COMPOUNDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CHOLESTEROL
CYCLOALKENES
DISTRIBUTION
DOMESTIC ANIMALS
DRUGS
ENDOTHELIUM
FLUORESCENCE
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
INDOLES
LABELLED COMPOUNDS
LIPIDS
LUMINESCENCE
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
MOLECULAR STRUCTURE
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
SEROTONIN
STEROIDS
STEROLS
SUBCELLULAR DISTRIBUTION
SWINE
SYMPATHOMIMETICS
TISSUES
TRYPTAMINES
VERTEBRATES