Genetic transformation and gene expression in white pine (pinus strobus)
Technical Report
·
OSTI ID:7049916
The objectives of the study were: (1) to develop protocols for transformation of white pine (Pinus strobus) embryonic tissue; and (2) to analyze the regulation of foreign gene expression in Pinus strobus. A number of Agrobacterium tumefaciens strains containing chimeric genes for neomycin phosphotransferase (NPTII for kanamycin resistance) and chloramphenicol acetyl transferase (CAT) under the control of either a constitutive promoter (NOS-nopaline synthase) or light-inducible promoters (RuBisCO small subunit and chlorophyll a/b binding protein) were used. A variety of tissues from white pine seedlings and mature trees was used. The techniques for transformation were modified from those used for tobacco transformation. The results show that white pine tissue from young seedlings is high suitable for transformation by A. tumefaciens. Whereas the normal tissues are very sensitive to kanamycin, transformed callus was quite resistant to this antibiotic.
- Research Organization:
- AETA Corp., Portsmouth, NH (United States)
- OSTI ID:
- 7049916
- Report Number(s):
- PB-94-159423/XAB; CNN: NSF-ISI-8660764
- Country of Publication:
- United States
- Language:
- English
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