Inheritance of a functional mouse metallothionein gene in tobacco

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6867779

Maiti, I B; Wagner, G J; Yeargan, R; Hunt, A G ^[1]

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1. Univ. of Kentucky, Lexington (USA)

Morphologically normal plants were obtained from progeny (Ro, R1 and R2) originating from tobacco leaf tissue transformed with Agrobacterium tumefaciens containing a chimeric gene for kanamycin resistance an the mouse metallothionein cDNA gene directed by the constitutive promote 35S from CaMV. Integration and expression in R1 progeny was demonstrated by Southern, Northern blot analysis and metallothionein assay. Kanamycin resistance analysis of R1 and R2 progeny showed inheritance to be as a dominant Mendelian trait. Seedlings obtained from self-fertilized transgenic tobacco are more tolerant to cadmium stress than nontransformed controls. Cadmium accumulation in leaves of transgenic seedlings exposed to a low, field-like Cd concentration was about 20% lower than that in nontransformed controls.

• OSTI ID: 6867779
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
CADMIUM
DOMINANT MUTATIONS
ELEMENTS
GENE REGULATION
GENES
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METALLOTHIONEIN
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PLANTS
PROTEINS
RODENTS
TOBACCO
TOXICITY
VERTEBRATES