Expression of mouse metallothionein genes in tobacco

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5839634

Maiti, I B; Yeargan, R; Wagner, G J; Hunt, A G ^[1]

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1. Univ. of Kentucky, Lexington (USA)

We have expressed a mouse metallothionein (NT) gene in tobacco under control of the cauliflower mosaic virus (CaMV) 35S promoter and a pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) gene promoter. Seedlings in which MT gene expression is driven by the 35S promoter are resistant to toxic levels of cadmium. Mature plants carrying the 35S-MT gene accumulate less Cd in their leaves when exposed to low levels of Cd in laboratory growth conditions. Plants with the rbcS-MT construction express this gene in a light-regulated and tissue-specific manner, as expected. Moreover, the MT levels in leaves in these plants are about 20% of those seen in 35S-MT plants. These plants are currently being tested for Cd resistance. In addition, a small field evaluation of 35S-MT lines for Cd levels is being evaluated. These experiments will address the possibility of using MTs to alter Cd levels in crop species.

• OSTI ID: 5839634
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CADMIUM
CARBON-CARBON LYASES
CARBOXY-LYASES
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LYASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICROORGANISMS
NICOTIANA
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PLANTS
PROTEINS
RADIOISOTOPES
RIBULOSE DIPHOSPHATE CARBOXYLASE
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TOXICITY
TRACER TECHNIQUES
VIRUSES