Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Effect of bacterial sepsis on gluconeogenic capacity in the rat

Journal Article · · J. Surg. Res.; (United States)
;
Since sepsis places increased demands on the host for energy and on other substrates for tissue repair and host defense, hepatic gluconeogenesis is critical for the host's adaptation to sepsis. Substrate-stimulated gluconeogenesis (i.e., gluconeogenic capacity) was assessed by the alanine load method in mannoheptulose-pretreated rats made septic by cecal ligation after laparotomy, as well as by cecal ligation and puncture after laparotomy. Fasted rats subjected to laparotomy only (sham-ligated) and fasted, nonoperated rats (controls) were investigated simultaneously. Following an overnight (-18 to 0 hr) fast, nonoperated animals converted 17.9 +/- 1.5% of (/sup 14/C)alanine to (/sup 14/C)glucose. Continued fasting in nonoperated animals resulted in enhanced (P less than 0.05) gluconeogenic capacity (6 hr = 27.2 +/- 3.0%; 24 hr = 26.2 +/- 1.9%; and 48 hr = 28.5 +/- 2.6%) relative to Time 0. Laparotomy alone (sham ligation) delayed the fasting-induced increase (P less than 0.05) in gluconeogenesis capacity (6 hr = 21.1 +/- 1.2%; 24 hr = 18.5 +/- 1.3%; 48 hr = 27.8 +/- 1.0%) relative to Time 0. In contrast, postoperative sepsis produced a sustained depression (P less than 0.05) of gluconeogenic capacity relative to nonoperated sham-ligated controls at 48 hr (cecal ligation, 18.4 +/- 1.4%; and cecal ligation and puncture, 18.8 +/- 1.2%). Thus, (1) fasting enhances hepatic gluconeogenic capacity; (2) surgical trauma transiently blunts the gluconeogenic response to fasting; and (3) sepsis undermines the gluconeogenic response to fasting.
Research Organization:
Albany Medical College of Union Univ., NY (USA)
OSTI ID:
7037901
Journal Information:
J. Surg. Res.; (United States), Journal Name: J. Surg. Res.; (United States) Vol. 45:2; ISSN JSGRA
Country of Publication:
United States
Language:
English

Similar Records

Studies on the possible role of thyroid hormone in altered muscle protein turnover during sepsis
Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Ann. Surg.; (United States) · OSTI ID:6326758
Effect of sepsis on calcium uptake and content in skeletal muscle and regulation in vitro by calcium of total and myofibrillar protein breakdown in control and septic muscle: Results from a preliminary study
Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Surgery; (United States) · OSTI ID:5816203
Normotensive sepsis is associated with increased lipid peroxidation products in skeletal muscle
Conference · Sun Feb 25 23:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States) · OSTI ID:6007088

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALANINES
ALDEHYDES
AMINO ACIDS
ANIMALS
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISEASES
FASTING
GLANDS
GLUCOSE
HEXOSES
HORMONES
INFECTIOUS DISEASES
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEDICINE
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RATS
RODENTS
SACCHARIDES
SURGERY
TRACER TECHNIQUES
VERTEBRATES