Normotensive sepsis is associated with increased lipid peroxidation products in skeletal muscle

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6007088

Lam, C; Fox, G; Neal, A; Webb, C; Rutledge, F; Myers, M; Sibbald, W ^[1]

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1. Victoria Hospital/UWO, London, Ontario (Canada)

Reactive oxygen species (ROS) have been implicated in the development of sepsis-induced multiple systems organ failure, possibly through biomembrane lipid perioxidation (BLP) which produces a loss of cell integrity and function. The authors examined the hypothesis that ROS activity contributes to non-pulmonary cell injury in hyperdynamic sepsis by measuring BLP products in skeletal muscle. The authors measured systemic flow (Q) by thermodilution and Q-gastrocnemius by the radioactive microsphere technique in 10 awake sheep, 48 hours following (i) the induction of hyperdynamic sepsis by cecal ligation and perforation or (ii) sham laparotomy. The animals were then anesthetized and biopsies from the gastrocnemius muscle were taken and flash frozen in liquid nitrogen for the determination of BLP products, which included conjugated dienes (CD), malondialdehyde (MDA), and acid-soluble sulfhydryls (SH). At the 48 hours study, Q was increased in the septic compared to the sham group while mean BP and Q-gastrocnemius were not different between the groups. Both CD and SH were significantly increased in the septic group. It was concluded that normotensive sepsis in this animal model produces evidence of increased ROS mediated BLP in non-pulmonary organs distant from the site of inflammation.

• OSTI ID: 6007088
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
ANIMALS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DOMESTIC ANIMALS
INFLAMMATION
LIPIDS
MAMMALS
MEMBRANES
MICROSPHERES
MUSCLES
ORGANIC COMPOUNDS
OXIDATION
PATHOLOGICAL CHANGES
RADICALS
RUMINANTS
SHEEP
SUPEROXIDE RADICALS
SYMPTOMS
TOXICITY
VERTEBRATES