Studies on the possible role of thyroid hormone in altered muscle protein turnover during sepsis

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Ann. Surg.; (United States)

DOI:https://doi.org/10.1097/00000658-198707000-00003· OSTI ID:6326758

Hasselgren, P O; Chen, I W; James, J H; Sperling, M; Warner, B W; Fischer, J E

Five days after thyroidectomy (Tx) or sham-Tx in young male Sprague-Dawley rats, sepsis was induced by cecal ligation and puncture (CLP). Control animals underwent laparotomy and manipulation of the cecum without ligation or puncture. Sixteen hours after CLP or laparotomy, protein synthesis and degradation were measured in incubated extensor digitorum longus (EDL) and soleus (SOL) muscles by determining rate of /sup 14/C-phenylalanine incorporation into protein and tyrosine release into incubation medium, respectively. Triiodothyronine (T3) was measured in serum and muscle tissue. Protein synthesis was reduced by 39% and 22% in EDL and SOL, respectively, 16 hours after CLP in sham-Tx rats. The response to sepsis of protein synthesis was abolished in Tx rats. Protein breakdown was increased by 113% and 68% in EDL and SOL, respectively, 16 hours after CLP in sham-Tx animals. The increase in muscle proteolysis during sepsis was blunted in hypothyroid animals and was 42% and 49% in EDL and SOL, respectively. T3 in serum was reduced by sepsis, both in Tx and sham-Tx rats. T3 in muscle, however, was maintained or increased during sepsis. Abolished or blunted response of muscle protein turnover after CLP in hypothyroid animals may reflect a role of thyroid hormones in altered muscle protein metabolism during sepsis. Reduced serum levels of T3, but maintained or increased muscle concentrations of the hormone, suggests that increased T3 uptake by muscle may be one mechanism of low T3 syndrome in sepsis, further supporting the concept of a role for thyroid hormone in metabolic alterations in muscle during sepsis.

• Research Organization: Univ. of Cincinnati, OH
• OSTI ID: 6326758
• Journal Information: Ann. Surg.; (United States), Journal Name: Ann. Surg.; (United States) Vol. 206:1; ISSN ANSUA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BACTERIAL DISEASES
BIOLOGICAL FUNCTIONS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
DISEASES
FUNCTIONS
HEMIC DISEASES
HORMONES
HYDROXY ACIDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEDICINE
METABOLISM
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PEPTIDE HORMONES
PHENYLALANINE
PROTEINS
PROTEOLYSIS
RATS
RODENTS
SEPTICEMIA
SURGERY
THYROID HORMONES
THYROIDECTOMY
TRACER TECHNIQUES
TRIIODOTHYRONINE
TYROSINE
VERTEBRATES