Isochores and CpG islands in YAC contigs in human Xq26. 1-qter

Journal Article · Sun Aug 01 04:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1347· OSTI ID:7035461

Pilia, G; Little, R D; Schlessinger, D ^[1]; Aiessani, B; Bernardi, G ^[2]

---

1. Washington Univ. School of Medicine, St. Louis, MO (United States)
2. Institut Jacques Monod, Paris (France)

GC levels were assessed at 37 loci across 30 Mb of Xq26.1-qter, a region physically mapped in overlapping yeast artificial chromosome clones. In 8 Mb of R band Xq26, GC is relatively high (up to 44T%) in the distal 4 Mb. Consistently low GC values (38-41%) are observed in G band Xq27. In contrast, further toward the telomere in Xq28, the GC level rises progressively to reach 52% at 2 to 4 Mb from the end of the chromosome; this region is delimited by low GC loci. Across these regions of Xq, the content of rare-cutter restriction enzyme sites containing CpG, including [open quotes]CpG islands[close quotes] in the most completely mapped Xq26-27.1 region, is correlated with GC level. Isochore mapping can thus provide one index of putative gene content across mapped regions. 43 refs., 5 figs.

• OSTI ID: 7035461
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINES
AROMATICS
AZAARENES
AZINES
CHROMOSOMES
CONCENTRATION RATIO
CYTOSINE
GUANINE
HETEROCHROMOSOMES
HETEROCYCLIC COMPOUNDS
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
HYDROXY COMPOUNDS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
PYRIMIDINES
X CHROMOSOME