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YAC/STS map across 12 Mb of Xq27 at 25-kb resolution, merging Xq26-qter

Journal Article · · Genomics
;  [1]; ;  [2]
  1. C.N.R.-I.T.B.A., Milano (Italy)
  2. Washington Univ. School of Medicine, St. Louis, MO (United States); and others
A 12-Mb YAC contig has been assembled spanning the Xq27 cytogenetic band with 203 YACs, 121 STSs, and >300 hybridization probes to a resolution of 25 kb. At its centromeric end, the contig is merged with a 9-Mb contig covering Xq26.1-q26.3 at a point 1 Mb telomeric to the factor IX gene; at its telomeric end, it is merged to 7.5 Mb of contigs form the IDS gene to the Xq28 telomere. Thus, the distal 29 Mb of the Xq arm is available cloned in long-range contiguity. The physical map has been integrated with current genetic data by the localization of 18 markers that detect polymorphism. Apparent recombination levels reach >4.5 cM/Mb near the centromeric border of Xq27. The ratio of cM/Mb correspondingly delimits the location of several disease genes-including, for example, X-linked hypoparathyroidism in 3 Mb (6 cM) telomeric to Factor IX. 50 refs., 3 figs., 1 tab.
OSTI ID:
478557
Journal Information:
Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 34; ISSN 0888-7543; ISSN GNMCEP
Country of Publication:
United States
Language:
English

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