Genotoxicity to human cells induced by air particulates isolated during the Kuwait oil fires

Journal Article · Sat Jan 01 00:00:00 EST 1994 · Environmental Research; (United States)

DOI:https://doi.org/10.1006/enrs.1994.1003· OSTI ID:7033558

Kelsey, K T; Xia, F; Christiani, D C; Liber, H L; Spengler, J D; Dockery, D W ^[1]; Bodell, W J ^[2]

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1. Harvard School of Public Health, Boston, MA (United States)
2. Univ. of California, San Francisco, CA (United States)

In an effort to examine the potential of exposure to soot from the 1991 oil fires in the Kuwait desert for inducing genetic effects we studied the in vitro genotoxicity of this materials. Air particulates isolated near the Kuwait oil fires were studied using three assays. Dose-dependent increases were observed for both sister chromatid exchanges in human peripheral blood lymphocytes and mutation at the hprt locus in the metabolically competent human lymphoblast cell line AHH-1. Similar magnitudes of response were seen using these two assays when testing a standard air particulate sample which had been isolated from the Washington, DC, area. Using the [sup 32]P-postlabeling assay, no increase in DNA adduct formation was observed in AHH-1 cells treated with particulates isolated from sampling in Kuwait. 18 refs., 4 figs.

• OSTI ID: 7033558
• Journal Information: Environmental Research; (United States), Vol. 64:1; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
02 PETROLEUM
OIL FIELDS
FIRES
SOOT
DOSE-RESPONSE RELATIONSHIPS
GENETIC EFFECTS
MUTATIONS
PARTICULATES
SISTER CHROMATID EXCHANGES
BIOLOGICAL EFFECTS
CHROMOSOMAL ABERRATIONS
GEOLOGIC DEPOSITS
MINERAL RESOURCES
PARTICLES
PETROLEUM DEPOSITS
RESOURCES
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry
020900 - Petroleum- Environmental Aspects