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Ligand binding and internalization by the rat hepatic asialoglycoprotein receptor does not generate polyphosphoinositide derived second messengers

Journal Article · · Life Sciences; (USA)
; ; ;  [1]
  1. Univ. of Texas Medical Branch, Galveston (USA)
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We have studied the effects of asialoorosomucoid (ASOR) on the hydrolysis of ({sup 32}P)-inositol phospholipids in isolated rat hepatocytes. When internalization of ASOR is maximal at 310 molecules/cell/sec, there is neither a decrease in the amount of ({sup 32}P)-phosphatidylinositol-4,5-bisphosphate (PIP{sub 2}) not an increase in ({sup 32}P)-phosphatidic acid (PA) up to 30 min after stimulation. On the other hand, 10-{sup 6}M vasopressin, which was used as a positive control, caused a 35-40% decrease in the level of ({sup 32}P)-PIP{sub 2} and a 70-80% increase in ({sup 32}P)-PA within 30 sec. Addition of orosomucoid or ASOR, even at concentrations 1000-times the K{sub d}, did not change the levels of any of the six phospholipids tested. Similarly, addition of ASOR did not increase the levels of soluble ({sup 3}H)-inositol phosphates, whereas vasopressin caused a 6-fold increase in ({sup 3}H)-inositol-1,4-diphosphate (IP{sub 2}) and a 4-fold increase in ({sup 3}H)-inositol-1,4,5-triphosphate (IP{sub 3}) in isolated rat hepatocytes prelabeled with ({sup 3}H)-inositol.
OSTI ID:
7027017
Journal Information:
Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 45:24; ISSN 0024-3205; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
ESTERS
GLYCOPROTEINS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGANDS
LIGHT NUCLEI
LIPIDS
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
METABOLISM
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES