Agonist induced formation of myoinositol 1,4,5-P/sub 3/, myoinositol 1,3,4-P/sub 3/ and myoinositol-P/sub 4/ in rat liver parenchymal cells

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6985820

Blackmore, P F; Bocckino, S; Jiang, H; Exton, J H

Treatment of hepatocytes with vasopressin (10/sup -7/ M) resulted in a rapid and large (approximately 7-fold) increase in myoinositol 1,4,5-P/sub 3/ (I 1,4,5-P/sub 3/) at 10 s which slowly declined over the next 30 s to reach a steady level (approximately 2-fold increase). IP/sub 4/ also increased (approximately 4-fold) at 10 s. The level of I 1,3,4-P/sub 3/ also increased approximately 100% at 2 min after a lag of approximately 20 s. The increase in I 1,4,5-P/sub 3/ correlates temporally with the increase in cytosolic free Ca/sup 2 +/. The dose response curves of vasopressin to increase I 1,4,5-P/sub 3/, I 1,3,4-P/sub 3/ and IP/sub 4/ measured at 1 min were similar. Other Ca/sup 2 +/ mobilizing agonists (ATP, angiotensin II, epinephrine, AlF/sub 4//sup -/ and glucagon) also increased I 1,4,5-P/sub 3/, I 1,3,4-P/sub 3/ and IP/sub 4/ to varying degrees. Incubation of permeabilized hepatocytes with (2-/sup 3/H)I 1,4,5-P/sub 3/ in the presence of MgATP resulted in the formation of (/sup 3/H)IP/sub 4/ and (/sup 3/H)I 1,3,4-P/sub 3/. Also I 1,4,5-P/sub 3/ was converted to (/sup 32/P)IP/sub 4/ in the presence of (..gamma..-/sup 32/P)ATP. Phosphorylation of I 1,4,5-P/sub 3/ to I 1,3,4,5-P/sub 4/, followed by dephosphorylation could result in the formation of I 1,3,4-P/sub 3/ and would account for the formation of these compounds following hormone stimulation of hepatocytes.

• Research Organization: Vanderbilt Univ., Nashville, TN
• OSTI ID: 6985820
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ADRENALINE
ALKALINE EARTH METAL COMPOUNDS
ANGIOTENSIN
ANIMAL CELLS
ANIMALS
ATP
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CALCIUM COMPOUNDS
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
GLOBULINS
HORMONES
INOSITOLS
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIVER CELLS
MAMMALS
MEMBRANE TRANSPORT
MONOSACCHARIDES
NEUROREGULATORS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VASOPRESSIN
VERTEBRATES