Dolichol alters brain membrane functions
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7024924
It has been well demonstrated that there is a direct correlation between increase in dolichol level in brain and aging. An abnormally high level of dolichol was found in brain tissue of patients with pathological aging disorders. The aim of this study is to examine the physiological significance of dolichol affecting membrane transport activity and phospholipid acyl group turnover. Dolichol added to synaptic plasma membranes resulted in a biphasic effect on (Na/sup +/, K/sup +/)-ATPase, i.e., an enhancement of activity at low concentrations (5 ..mu..g/125 mg protein) and an inhibition of activity at high concentrations (40-100 ..mu..g). To probe the membrane acyl group turnover, the incorporation of (/sup 14/C)-arachidonate into plasma membrane phospholipids was examined in the presence and absence of dolichol. Dolichol elicited an increase in the incorporation of label into phospholipids. However, the effects varied depending on whether BSA is present. In the absence of BSA, the increase in labeling of phosphatidylinositols is higher than that of phosphatidylcholines. These results suggest that dolichols, when inserted into membranes, may alter membrane functions.
- Research Organization:
- Univ. of Missouri, Columbia
- OSTI ID:
- 7024924
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ARACHIDONIC ACID
ATP-ASE
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MEMBRANE TRANSPORT
MEMBRANES
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ARACHIDONIC ACID
ATP-ASE
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
BRAIN
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MEMBRANE TRANSPORT
MEMBRANES
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
TRACER TECHNIQUES