Neomycin inhibits the phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate stimulation of plasma membrane ATPase activity

Journal Article · Wed May 01 04:00:00 EDT 1991 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.96.1.340· OSTI ID:7111279

Chen, Qiuyun; Boss, W F ^[1]

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1. North Carolina State Univ., Raleigh (United States)

The inositol phospholipids, phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP{sub 2}), have been shown to increase the vanadate-sensitive ATPase activity of plant plasma membranes. In this paper, the authors show the effect of various concentrations of phosphatidyinositol, PIP, and PIP{sub 2} on the plasma membrane vanadate-sensitive ATPase activity. PIP and PIP{sub 2} at concentrations at 10 nanomoles per 30 microgram membrane protein per milliliter of reaction mixture caused a twofold and 1.8-fold increase in the ATPase activity, respectively. The effect of these negatively charged phospholipids on the ATPase activity was inhibited by adding the positively charged aminoglycoside, neomycin. Neomycin did not affect the endogenous plasma membrane ATPase activity in the absence of exogenous lipids.

• OSTI ID: 7111279
• Journal Information: Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 96:1; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ATP-ASE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
HYDROLASES
INHIBITION
KINETICS
LIPIDS
MEMBRANES
NEOMYCIN
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHOLIPIDS
PLANT CELLS
PROTEINS
REACTION KINETICS
STIMULATION
TRANSITION ELEMENT COMPOUNDS
VANADATES
VANADIUM COMPOUNDS