Phosphatidylinositol turnover is associated with human natural killer cell activation by tumor target cells

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5276912

Steele, T A; Brahmi, Z

Natural Killer (NK) cell activity has been shown to be a binding-dependent event leading to the destruction of various targets. This suggests a possible role for plasma membrane phospholipid turnover in coupling a receptor-mediated binding event with transduction of a intracellular signal to result in the activation of the effector cell. Currently, phosphatidylinositol (PI) turnover is implicated in several immune cell systems. Therefore, in this study, the authors examined phospholipid turnover in human NK cells upon exposure to a sensitive (K562) and a resistant (YAC-1) target cell (TC). NK cell membrane phospholipids were labelled with Phosphorus-32 (/sup 32/P) and, following stimulation, were extracted and run on silica gel thin-layer chromatography. Labelled phospholipids were visualized by autoradiography then scraped and counted in a liquid scintillation counter. A 2.5 fold increase in label incorporation into PI relative to controls was shown to occur when NK cells were stimulated by K562 for 2 hours. In contrast, no increased labelling of PI relative to controls was noted when NK cells were stimulated by YAC-1 for the same period of time. No change in incorporation of /sup 32/P into phosphatidylcholine or phosphatidylethanolamine occurred in either set of conditions. These results suggest that PI turnover may be an early activation event in NK cells following binding of K562.

• Research Organization: Indiana Univ. School of Medicine, Indianapolis
• OSTI ID: 5276912
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CELL PROLIFERATION
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ESTERS
INOSITOL
INOSITOLS
ISOTOPES
KINETICS
LABELLING
LEUKOCYTES
LIGHT NUCLEI
LIPIDS
LYMPHOCYTES
MATERIALS
MEMBRANES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
THIN-LAYER CHROMATOGRAPHY
TUMOR CELLS