C-reactive protein bearing cells are a subpopulation of natural killer cell precursors

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6763994

Baum, L L; Krueger, N X

Cell surface C-reactive protein (S-CRP) is expressed on the surface membrane of a small percentage of lymphocytes. Anti-CRP inhibits natural killer (NK) function. Since NK effectors are heterogeneous, they suspected that the cells expressing S-CRP (CRP/sup +/) might respond differently to stimulation than the NK effectors lacking S-CRP (CRP/sup -/). Methods were developed to separate CRP/sup +/ and CRP/sup -/ lymphocytes and their functional responses were examined and compared. These techniques are dependent upon the binding of CRP to its ligands, C-polysaccharide (CPS) or Phosphocholine (PC). The first method involves rosette formation with CPS coupled autologous red blood cells; the second method utilizes the binding of CRP/sup +/ lymphocytes to PC-sepharose. Lymphocytes separated using either of these techniques yield similar results. CRP/sup -/ lymphocytes respond to 3 day incubation with PHA or Il-2 by producing effectors which kill /sup 51/Cr labeled K562 tumor cells, CRP/sup +/ precursors do not. CRP/sup +/ lymphocytes respond to a 5 day incubation with inactivated K562 by producing effectors which kill K562; CRP/sup -/ precursors do not. NK functional activity of both is increased by incubation with interferon. This ability to respond differently to stimulation suggests that CRP/sup +/ and CRP/sup -/ cells are functionally distinct.

• Research Organization: Chicago Medical School, IL
• OSTI ID: 6763994
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL KILLING
CELL MEMBRANES
CHEMICAL COMPOSITION
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANES
NUCLEI
ORGANIC COMPOUNDS
PRECURSOR
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
TRACER TECHNIQUES
TUMOR CELLS