Radiosensitivity of human natural killer cells: Binding and cytotoxic activities of natural killer cell subsets

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577701· OSTI ID:6271530

Rana, R; Vitale, M; Mazzotti, G; Manzoli, L; Papa, S ^[1]

---

1. Universita di Bologna (Italy)

The sensitivity of human natural killer (NK) cell activities (both binding and killing) after exposure of peripheral blood mononuclear cells to different doses of gamma radiation was studied. A panel of monoclonal antibodies was used to identify the NK and T-lymphocyte subsets and to evaluate their radiosensitivity. Peripheral blood mononuclear cells were irradiated with low (2-6 Gy) and high (10-30 Gy) doses and NK cell binding and cytotoxic activity against K562 target cells were studied after 3 h and 48 h in culture. The primary damage to NK cell activity was identified at the postbinding level and affected mainly the lytic machinery. After 48 h culture postirradiation, an overall depression of cytotoxic activity was observed, but ionizing radiation produced either a selection of the more cytotoxic NK cell subsets, which therefore might be considered more resistant to radiation damage than the less cytotoxic NK cells, or a long-term stimulation of cytotoxic activity in surviving cells.

• OSTI ID: 6271530
• Journal Information: Radiation Research; (USA), Vol. 124:1; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
RADIOSENSITIVITY
MONOCYTES
BIOLOGICAL RADIATION EFFECTS
GAMMA RADIATION
MAN
MONOCLONAL ANTIBODIES
PHENOTYPE
RADIATION DOSES
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DOSES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
MAMMALS
MATERIALS
PRIMATES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture