Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Effects of X-ray irradiation on natural killer (NK) cell system. II. Increased sensitivity to natural killer cytotoxic factor (NKCF)

Journal Article · · Immunopharmacology and Immunotoxicology; (USA)
; ; ;  [1]
  1. Kyoto Univ. (Japan)
+ Show Author Affiliations

Irradiation with low-doses of X-rays of tumor cells elevated their susceptibility to lysis by natural killer (NK) cells in an accompanying paper. Cytotoxicity assays conducted at the single cell level revealed that X-ray irradiation of K562 cells did not affect the number of effector-target conjugates but increased the frequency of dead conjugated target cells. During interaction with K562 cells large granular lymphocytes released a soluble cytotoxic factor (NKCF) that killed the target cells. X-ray irradiation did not affect the NKCF stimulatory ability of K562 cells, while it elevated their sensitivity to the lytic effect of NKCF. In contrast to X-rays, exposure to ultraviolet (UV) radiation of K562 cells did not elevate their NK sensitivity but rather reduced it. Treatment with mitomycin C produced no effect on NK sensitivity. These results indicate that X-ray irradiation elevates the target sensitivity to NKCF, which may be involved in the increased NK sensitivity, and that the X-ray effect may be different from that of UV radiation or DNA synthesis inhibition.

OSTI ID:
6992753
Journal Information:
Immunopharmacology and Immunotoxicology; (USA), Journal Name: Immunopharmacology and Immunotoxicology; (USA) Vol. 11:2-3; ISSN 0892-3973; ISSN IITOE
Country of Publication:
United States
Language:
English

Similar Records

Killing defect of natural killer cells with the absence of natural killer cytotoxic factors in a child with Hodgkin's disease
Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Blood; (United States) · OSTI ID:6274990
Effects of X-ray irradiation on natural killer (NK) cell system. I. Elevation of sensitivity of tumor cells and lytic function of NK cells
Journal Article · Sat Dec 31 23:00:00 EST 1988 · Immunopharmacology and Immunotoxicology; (USA) · OSTI ID:6937414
Radiosensitivity of human natural killer cells: Binding and cytotoxic activities of natural killer cell subsets
Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Radiation Research; (USA) · OSTI ID:6271530

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
LYSIS
MATERIALS
MITOMYCIN
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
TUMOR CELLS
ULTRAVIOLET RADIATION
X RADIATION