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Immunologic and biochemical detection of cell surface alterations following morphological transformation or mutation of C3H/10T1/2 C1 8 cells by chemical carcinogens

Book ·
OSTI ID:7023993

The C3H/10T1/2 C1 in mouse embryo fibroblast cell line is a permanent, cultured line whose hypotetraploid cells are contact inhibited and nontumorigenic in immunosuppressed syngeneic mice. Upon treatment with certain chemical carcinogens or radiation, some of these cells become morphologically transformed so that they are no longer contact inhibited and form foci, which are tumorigenic in immunosuppressed, syngeneic mice. Resistance to the cardiac glycoside ouabain can also be chemically induced in these cells, presumably by mutation of the gene encoding the Na/sup +/, K/sup +/-dependent adenosinetriphosphatase cation transport enzyme. Thus C3H/10T1/2 C1 8 cells find utility as in vitro neoplastic transformation and mutation systems. Morphologic transformation induced by chemical carcinogens in C3H/10T1/2 cells is accompanied by complex antigenic changes. These changes were studied using /sup 125/I labelled Protein A IRMA and fluorescence-activated cell sorter immunofluorescence assays. A panel of thirty different polyclonal antisera was prepared against transformed and nontransformed C3H/10T1/2 cell lines. Mutation of the gene encoding the (Na,K)-ATPase enzyme is hypothesized to be responsible for the chemical carcinogen inducation of the ouabain resistance (Oua/sup r/) phenotype in C3H/10T1/2 cell lines. In order to test this hypothesis, the (Na,K)-ATPase enzyme activity of wild type and Oua/sup r/ C3H/10T1/2 cells were characterized for resistance to ouabain.

Research Organization:
University of Southern California, Los Angeles (USA)
OSTI ID:
7023993
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560300 -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ANIMAL CELLS
ATP-ASE
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARCINOGENESIS
CARCINOGENS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EMBRYONIC CELLS
ENZYME ACTIVITY
ENZYMES
FIBROBLASTS
FLUORESCENCE
GLYCOSIDES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
MEMBRANES
MUTATIONS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
OUABAIN
PATHOGENESIS
PHOSPHOHYDROLASES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SOMATIC CELLS
STROPHANTHINS
TRACER TECHNIQUES