Morphological and neoplastic transformation of C3H/10T1/2 Cl 8 mouse embryo cells by insoluble carcinogenic nickel compounds

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Environmental and Molecular Mutagenesis; (USA)

DOI:https://doi.org/10.1002/em.2850140202· OSTI ID:6833015

Miura, T; Patierno, S R; Sakuramoto, T; Landolph, J R ^[1]

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1. Univ. of Southern California School of Medicine, Los Angeles (USA)

The authors studied induction of cytotoxicity and morphological transformation in C3H/10T1/2 Cl8 (10T1/2) mouse embryo fibroblasts by soluble and insoluble carcinogenic nickel compounds. Soluble nickel sulfate and nickel chloride caused dose-dependent cytotoxicity after 48 hr treatments, but neither compound induced morphological transformation even at concentrations causing up to 94% cytotoxicity. In soluble nickel subsulfide, nickel monosulfide, and nickel oxide caused dose-dependent cytotoxicity and a low, dose-dependent frequency of morphological transformation after 48 hr exposure of cells to these compounds. Foci were predominantly of type II morphology; type III foci were rare. The insoluble nickel compounds studied caused no induction of base substitution mutations to ouabain resistance in 10T1/2 cells over concentration ranges that induced morphological transformation. They twice cloned type II and type III foci induced by insoluble nickel compounds, established independent cell lines, and characterized their phenotypes. These data indicate that insoluble carcinogenic nickel compounds induced type II foci in 10T1/2 cells, some of which were tumorigenic, and that the 10T1/2 cell system is suitable for studying mechanisms of nickel compound-induced morphological transformation in mammalian cells.

• OSTI ID: 6833015
• Journal Information: Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 14:2; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL TRANSFORMATIONS
CHLORIDES
CHLORINE COMPOUNDS
CONNECTIVE TISSUE CELLS
CYTOLOGY
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EMBRYONIC CELLS
FIBROBLASTS
GLYCOSIDES
HALIDES
HALOGEN COMPOUNDS
MAMMALS
MICE
MORPHOLOGICAL CHANGES
MUTATION FREQUENCY
NICKEL CHLORIDES
NICKEL COMPOUNDS
NICKEL SULFATES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
OUABAIN
OXYGEN COMPOUNDS
PHAGOCYTOSIS
RODENTS
SOMATIC CELLS
STROPHANTHINS
SULFATES
SULFUR COMPOUNDS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES